Authentication of shiitake powder using HPLC fingerprints combined with chemometrics

From the paper

Abstract

Shiitake powder is an important part of international agricultural trade. It is generally in dry powder state due to the requirement of preservation, so economically motivated adulteration in the shoddy way (using stipe instead of pileus) or the counterfeit way (using cheap mushroom) should be concerned, while methods for the authenticity identification of shiitake powder were barely reported. Herein, a fingerprint method was developed for the authenticity identification of shiitake powder through high-performance liquid chromatography (HPLC) coupled with chemometrics. The results showed that shiitake pileus powder and stipe powder can be discriminated using PCA and OPLS-DA. Besides, shiitake pileus powder, Pleurotus eryngii (P. eryngii) powder, shiitake cultivation bag powder and Pleurotus ostreatus (P. ostreatus) powder can be discriminated using OPLS-DA. This work provided usable technical solutions for two ways of economically motivated adulteration in shiitake powder.