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Maitake, Hen of the Woods · 1998 · Journal Article

Medium relevance

Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Escherichia coli.

Grifola frondosa

Immune support
SpeciesMaitake, Hen of the Woods
JournalApplied microbiology and biotechnology
Year1998

Key points

  • The genomic DNA and cDNA for a gene encoding a novel trehalose synthase (TSase) catalyzing trehalose synthesis from alpha-D-glucose 1-phosphate and D-glucose were cloned from a basidiomycete, Grifola frondosa
  • Nucleotide sequencing showed that the 732-amino-acid TSase-encoding region was separated by eight introns
  • Consistent with the novelty of TSase, there were no homologous proteins registered in the data-bases
  • Recombinant TSase with a histidine tag at the NH2-terminal end, produced in Escherichia coli, showed enzyme activity similar to that purified from the original G. frondosa strain
  • Incubation of alpha-D-glucose 1-phosphate and D-glucose in the presence of recombinant TSase generated trehalose, in agreement with the enzymatic property of TSase that the equilibrium lay far in the direction of trehalose synthesis

Metadata-grounded summary

Citation abstract

The genomic DNA and cDNA for a gene encoding a novel trehalose synthase (TSase) catalyzing trehalose synthesis from alpha-D-glucose 1-phosphate and D-glucose were cloned from a basidiomycete, Grifola frondosa. Nucleotide sequencing showed that the 732-amino-acid TSase-encoding region was separated by eight introns. Consistent with the novelty of TSase, there were no homologous proteins registered in the data-bases. Recombinant TSase with a histidine tag at the NH2-terminal end, produced in Escherichia coli, showed enzyme activity similar to that purified from the original G. frondosa strain. Incubation of alpha-D-glucose 1-phosphate and D-glucose in the presence of recombinant TSase generated trehalose, in agreement with the enzymatic property of TSase that the equilibrium lay far in the direction of trehalose synthesis.

Citation

Saito K, Yamazaki H, Ohnishi Y, Fujimoto S, Takahashi E, Horinouchi S (1998). Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Escherichia coli. Applied microbiology and biotechnology https://doi.org/10.1007/s002530051276 PMID: 9763690

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