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King Oyster, Eryngii · 1999 · Research Support, Non U.S. Gov'T

Low relevance

Aa-Pri2, a single-copy gene from Agrocybe aegerita, specifically expressed during fruiting initiation, encodes a hydrophobin with a leucine-zipper domain.

Pleurotus eryngii

Gut & microbiome
SpeciesKing Oyster, Eryngii
JournalCurrent genetics
Year1999

Key points

  • The Aa-Pri2 gene, specifically expressed during basidiocarp differentiation of the mushroom Agrocybe aegerita, was cloned
  • Sequence analysis showed a 525-nt ORF interrupted by three introns (51, 48, 54 bp)
  • One TATA box (-94), two CAAT boxes (-382, -398) and one CT-rich motif (-149) were present
  • The transcription start point was located by primer extension at position -60
  • The hydrophobic Aa-PRI2 protein (123 aa) possessed a putative signal peptide (aa1-aa18), eight cysteine residues characteristic of the hydrophobin proteins, and one putative leucine-zipper domain (aa50-aa71)
  • Hybridization of the genomic DNA of several edible mushrooms with the Aa-Pri2 cDNA, showed a 2.7-kb DNA fragment highly similar to the Aa-Pri2 gene in the species Agrocybe chaxingu; sequences showing slight hybridization signals were detected in Pleurotus eryngii, Lentinula edodes and Agaricus bisporus

Metadata-grounded summary

Citation abstract

The Aa-Pri2 gene, specifically expressed during basidiocarp differentiation of the mushroom Agrocybe aegerita, was cloned. Sequence analysis showed a 525-nt ORF interrupted by three introns (51, 48, 54 bp). One TATA box (-94), two CAAT boxes (-382, -398) and one CT-rich motif (-149) were present. The transcription start point was located by primer extension at position -60. The hydrophobic Aa-PRI2 protein (123 aa) possessed a putative signal peptide (aa1-aa18), eight cysteine residues characteristic of the hydrophobin proteins, and one putative leucine-zipper domain (aa50-aa71). Hybridization of the genomic DNA of several edible mushrooms with the Aa-Pri2 cDNA, showed a 2.7-kb DNA fragment highly similar to the Aa-Pri2 gene in the species Agrocybe chaxingu; sequences showing slight hybridization signals were detected in Pleurotus eryngii, Lentinula edodes and Agaricus bisporus.

Citation

Santos C, Labarère J (1999). Aa-Pri2, a single-copy gene from Agrocybe aegerita, specifically expressed during fruiting initiation, encodes a hydrophobin with a leucine-zipper domain. Current genetics https://doi.org/10.1007/s002940050454 PMID: 10369965

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