Turkey Tail, Yun Zhi · 2008 · Research Support, Non U.S. Gov'T
Medium relevancePurification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye.
Trametes versicolor
Key points
- A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium
- The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration
- The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used
- The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators
Metadata-grounded summary
Citation abstract
A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.
Citation
Guo M, Lu F, Liu M, Li T, Pu J, Wang N, et al. (2008). Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye. Biotechnology letters https://doi.org/10.1007/s10529-008-9817-z PMID: 18688574
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