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Turkey Tail, Yun Zhi · 2015 · Research Support, Non U.S. Gov'T

Medium relevance

Production, purification and biochemical characterization of two laccase isoforms produced by Trametes versicolor grown on oak sawdust.

Trametes versicolor

OncologyImmune support
SpeciesTurkey Tail, Yun Zhi
JournalBiotechnology letters
Year2015

Key points

  • Two laccase isoforms (lcc1 and lcc2) produced by Trametes versicolor, grown on oak sawdust under solid-state fermentation conditions, were purified and characterized
  • The two isoforms showed significant biochemical differences
  • Lcc1 and lcc2 had MWs of 60 and 100 kDa, respectively
  • Both isoforms had maximal activity at pH 3 with ABTS and 2,6-dimethyloxyphenol (DMP)
  • Lcc1 was the most attractive isoform due to its greater affinity towards all the laccase substrates used
  • Lcc2 had equivalent values of 45, 47, 15 and 39 mM. The biochemical properties of lcc1 substantiate the potential of this enzyme for application in the treatment of contaminated water with low pH values and high phenolic content

Metadata-grounded summary

Citation abstract

Two laccase isoforms (lcc1 and lcc2) produced by Trametes versicolor, grown on oak sawdust under solid-state fermentation conditions, were purified and characterized. The two isoforms showed significant biochemical differences. Lcc1 and lcc2 had MWs of 60 and 100 kDa, respectively. Both isoforms had maximal activity at pH 3 with ABTS and 2,6-dimethyloxyphenol (DMP). Lcc1 was the most attractive isoform due to its greater affinity towards all the laccase substrates used. Lcc1 had Km values of 12, 10, 15 and 17 mM towards ABTS, DMP, guaiacol and syringaldazine, respectively. Lcc2 had equivalent values of 45, 47, 15 and 39 mM. The biochemical properties of lcc1 substantiate the potential of this enzyme for application in the treatment of contaminated water with low pH values and high phenolic content.

Citation

Martínez-Morales F, Bertrand B, Pasión Nava AA, Tinoco R, Acosta-Urdapilleta L, Trejo-Hernández MR (2015). Production, purification and biochemical characterization of two laccase isoforms produced by Trametes versicolor grown on oak sawdust. Biotechnology letters https://doi.org/10.1007/s10529-014-1679-y PMID: 25257594

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