Back to search

Snow Fungus, Silver Ear · 2015 · Research Support, Non U.S. Gov'T

Medium relevance

Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg.

Tremella fuciformis

Gut & microbiomeLiver supportSkin & hydration
SpeciesSnow Fungus, Silver Ear
JournalBiotechnology letters
Year2015

Key points

  • Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter
  • Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization
  • Immunoblotting verified expression of the recombinant protein
  • Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters

Metadata-grounded summary

Citation abstract

Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters.

Citation

Shin DI, Song KS, Park HS (2015). Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg. Biotechnology letters https://doi.org/10.1007/s10529-014-1720-1 PMID: 25374008

Open citation