Turkey Tail, Yun Zhi · 2016 · Research Support, Non U.S. Gov'T
High relevanceA novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor.
Trametes versicolor
Key points
- OBJECTIVES: To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source
- RESULTS: Trametes versicolor 1017 produces two laccase isoenzymes with a total activity of 10 U ml(-1) within 8 days when using wheat bran and peanut powder as energy sources in liquid culture medium
- A novel isoenzyme, named Tvlac, was identified, purified and characterized
- Its optimum pH and temperature were from 4.5 to 5 and 55 to 60 °C, respectively
- Its activity was stimulated by ethanol at 10 % (v/v) which increased the V 0
- CONCLUSIONS: The biochemical properties of Tvlac substantiate the potential of this enzyme for applications under an aqueous ethanol mixture environment
Metadata-grounded summary
Citation abstract
OBJECTIVES: To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source.
RESULTS: Trametes versicolor 1017 produces two laccase isoenzymes with a total activity of 10 U ml(-1) within 8 days when using wheat bran and peanut powder as energy sources in liquid culture medium. A novel isoenzyme, named Tvlac, was identified, purified and characterized. Its optimum pH and temperature were from 4.5 to 5 and 55 to 60 °C, respectively. Its activity was stimulated by ethanol at 10 % (v/v) which increased the V 0.
CONCLUSIONS: The biochemical properties of Tvlac substantiate the potential of this enzyme for applications under an aqueous ethanol mixture environment.
Citation
Chen L, Yi X, Deng F, Fang W, Zhang X, Wang X, et al. (2016). A novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor. Biotechnology letters https://doi.org/10.1007/s10529-015-1994-y PMID: 26553026
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