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Shiitake, Hua Gu · 2011 · Journal Article

High relevance

Development of IRAP-SCAR marker for strain identification in Lentinula edodes

Lentinula edodes

SpeciesShiitake, Hua Gu
JournalWorld journal of microbiology & biotechnology
Year2011

Key points

  • Rapid and correct authentication of commercial strains is still important in today’s mushroom industry
  • Here for the first time we reported the using of sequence characterized amplified region (SCAR) marker developed from inter-retrotransposon amplified polymorphism (IRAP) marker to identify Lentinula edodes strain
  • Genomic polymorphism among 44 shiitake cultivars in China were surveyed by 24 IRAP primer combinations, from which primer combination LTR1L/MarY1R could generate a unique and reproducible 1712Â bp fragment to distinguish strain No. 4 from other 43 strains
  • Based on this strain-specific fragment, a SCAR primer pair was designed and its validity was verified by correctly amplifying a single strain-specific fragment from DNA samples of 100 L. edodes strains
  • Our study lays the foundation for developing strain-specific SCAR marker by retrotransposon-based marker technique in fungi

From the paper

Abstract

Rapid and correct authentication of commercial strains is still important in today’s mushroom industry. Here for the first time we reported the using of sequence characterized amplified region (SCAR) marker developed from inter-retrotransposon amplified polymorphism (IRAP) marker to identify Lentinula edodes strain. Genomic polymorphism among 44 shiitake cultivars in China were surveyed by 24 IRAP primer combinations, from which primer combination LTR1L/MarY1R could generate a unique and reproducible 1712 bp fragment to distinguish strain No. 4 from other 43 strains. Based on this strain-specific fragment, a SCAR primer pair was designed and its validity was verified by correctly amplifying a single strain-specific fragment from DNA samples of 100 L. edodes strains. Our study lays the foundation for developing strain-specific SCAR marker by retrotransposon-based marker technique in fungi.

Citation

Xiao Y, Dai Y, Lu Y, Liu W, Wang Z, Bian Y (2011). Development of IRAP-SCAR marker for strain identification in Lentinula edodes. World journal of microbiology & biotechnology https://doi.org/10.1007/s11274-010-0626-x

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