King Oyster, Eryngii · 2010 · Research Support, Non U.S. Gov'T
High relevanceGenetic introduction of foreign genes to Pleurotus eryngii by restriction enzyme-mediated integration.
Pleurotus eryngii
Key points
- Pleurotus eryngii was transformed via restriction enzyme-mediated integration
- In order to construct the transformation plasmid, the enhanced cyan fluorescent protein (ECFP) gene was ligated next to the gpd promoter of the plasmid pAN7-1
- Transformation was facilitated via the heat treatment of a transformation mixture containing 1 microg of the HindIII-digested plasmid DNA and 10(6) mushroom protoplasts in 40% polyethyleneglycol solution, resulting in 10-40 hygromycin-resistant transformants
- Successful transformation was evidenced by PCR, Southern blot, and confocal fluorescence microscopic analyses on the selected transformants
- To date, this is the first report on the transformation of P. eryngii by REMI technique
Metadata-grounded summary
Citation abstract
Pleurotus eryngii was transformed via restriction enzyme-mediated integration. In order to construct the transformation plasmid, the enhanced cyan fluorescent protein (ECFP) gene was ligated next to the gpd promoter of the plasmid pAN7-1. Transformation was facilitated via the heat treatment of a transformation mixture containing 1 microg of the HindIII-digested plasmid DNA and 10(6) mushroom protoplasts in 40% polyethyleneglycol solution, resulting in 10-40 hygromycin-resistant transformants. Successful transformation was evidenced by PCR, Southern blot, and confocal fluorescence microscopic analyses on the selected transformants. To date, this is the first report on the transformation of P. eryngii by REMI technique.
Citation
Noh W, Kim SW, Dong-Won B, Kim JY, Ro HS (2010). Genetic introduction of foreign genes to Pleurotus eryngii by restriction enzyme-mediated integration. Journal of microbiology (Seoul, Korea) https://doi.org/10.1007/s12275-010-9278-7 PMID: 20437160
Open citation