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Turkey Tail, Yun Zhi · 2012 · Research Support, Non U.S. Gov'T

Medium relevance

Removal of monomer delignification products by laccase from Trametes versicolor.

Trametes versicolor

OncologyImmune support
SpeciesTurkey Tail, Yun Zhi
JournalBioresource technology
Year2012

Key points

  • The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined
  • Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5 U/mL laccase whilst higher dosage showed no improvement
  • Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24h
  • However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase
  • Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1 day (LRG-B), no effect of laccase (LRG-C)
  • Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions

Metadata-grounded summary

Citation abstract

The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5 U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1 day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.

Citation

Kolb M, Sieber V, Amann M, Faulstich M, Schieder D (2012). Removal of monomer delignification products by laccase from Trametes versicolor. Bioresource technology https://doi.org/10.1016/j.biortech.2011.11.080 PMID: 22176974

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