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Maitake, Hen of the Woods · 2012 · Research Support, Non U.S. Gov'T

Low relevance

Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, Chlorophyllum molybdites.

Grifola frondosa

Immune support
SpeciesMaitake, Hen of the Woods
JournalBioorganic & medicinal chemistry
Year2012

Key points

  • A toxic protein, dubbed molybdophyllysin, was isolated from the tropical toadstool Chlorophyllum molybdites by following its lethal effect in mice
  • Analysis of the protein using SDS-PAGE revealed a single 23-kDa band
  • Sequence analysis of molybdophyllysin tryptic fragments showed that this protein is highly homologous to metalloendopeptidases (MEPs) obtained from edible mushrooms, such as Grifola frondosa, Pleurotus ostreatus, and Armillaria mellea
  • These proteins include a HEXXH+D zinc-binding motif known as aspzincin
  • Molybdophyllysin retained its proteolytic activity at temperatures up to 60°C with an optimum pH of 7.0
  • The activity was inhibited by both 1,10-phenanthroline and N-bromosuccinimide, but molybdophyllysin exhibited strong resistance to SDS

Metadata-grounded summary

Citation abstract

A toxic protein, dubbed molybdophyllysin, was isolated from the tropical toadstool Chlorophyllum molybdites by following its lethal effect in mice. Analysis of the protein using SDS-PAGE revealed a single 23-kDa band. Sequence analysis of molybdophyllysin tryptic fragments showed that this protein is highly homologous to metalloendopeptidases (MEPs) obtained from edible mushrooms, such as Grifola frondosa, Pleurotus ostreatus, and Armillaria mellea. These proteins include a HEXXH+D zinc-binding motif known as aspzincin. Accordingly, molybdophyllysin is a member of the deuterolysin family of zinc proteases. Molybdophyllysin retained its proteolytic activity at temperatures up to 60°C with an optimum pH of 7.0. The activity was inhibited by both 1,10-phenanthroline and N-bromosuccinimide, but molybdophyllysin exhibited strong resistance to SDS.

Citation

Yamada M, Tokumitsu N, Saikawa Y, Nakata M, Asano J, Miyairi K, et al. (2012). Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, Chlorophyllum molybdites. Bioorganic & medicinal chemistry https://doi.org/10.1016/j.bmc.2012.09.036 PMID: 23063519

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