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Maitake, Hen of the Woods · 2009 · Journal Article

Low relevance

A method for terminus proteomics: selective isolation and labeling of N-terminal peptide from protein through transamination reaction.

Grifola frondosa

Immune support
SpeciesMaitake, Hen of the Woods
JournalBioorganic & medicinal chemistry letters
Year2009

Key points

  • A novel method for selectively labeling and isolating N-terminal peptide from protein has been developed
  • An N(alpha)-amino group of protein was converted to a carbonyl group through transamination reaction and the resulting carbonyl group was modified with O-(4-nitrobenzyl)hydroxylamine (NBHA)
  • After proteolytic digestion using Grifola frondosa metalloendopeptidase (LysN), the modified N-terminal peptide remained unbound in the following treatment using amino-reactive p-phenylenediisothiocyanate (DITC) glass, whereas peptides other than the N-terminal peptide were effectively scavenged from the supernatant solution
  • The modified N-terminal peptide was thus successfully isolated and sequenced by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS) analysis

Metadata-grounded summary

Citation abstract

A novel method for selectively labeling and isolating N-terminal peptide from protein has been developed. An N(alpha)-amino group of protein was converted to a carbonyl group through transamination reaction and the resulting carbonyl group was modified with O-(4-nitrobenzyl)hydroxylamine (NBHA). After proteolytic digestion using Grifola frondosa metalloendopeptidase (LysN), the modified N-terminal peptide remained unbound in the following treatment using amino-reactive p-phenylenediisothiocyanate (DITC) glass, whereas peptides other than the N-terminal peptide were effectively scavenged from the supernatant solution. The modified N-terminal peptide was thus successfully isolated and sequenced by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS) analysis.

Citation

Sonomura K, Kuyama H, Matsuo E, Tsunasawa S, Nishimura O (2009). A method for terminus proteomics: selective isolation and labeling of N-terminal peptide from protein through transamination reaction. Bioorganic & medicinal chemistry letters https://doi.org/10.1016/j.bmcl.2009.10.044 PMID: 19864134

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