Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium

Flammulina velutipes

OncologyImmune supportLiver support

SpeciesEnoki, Winter Mushroom

JournalCarbohydrate polymers

Year2007

Key points

A water-soluble polysaccharide named as FVP2 has been isolated from Flammulina velutipes mycelium by hot water extraction, anion-exchange and gel-permeation chromatography
Structure study shows FVP2 is an α-(1[rightward arrow]4)-d-glucan, with a single α-d-glucan at the C-6 position approximately every seven residues, along the main chain
The weight-average molecular weight is 1.89 x 10⁴ Da
Its biological activity was tested on hepatocytes
In vitro study indicates FVP2 can enhance the livability of primary culture of mouse hepatocytes and decrease the release of ALT as well as apoptosis of hepatocytes after CCl₄ intoxication

From the paper

Abstract

A water-soluble polysaccharide named as FVP2 has been isolated from Flammulina velutipes mycelium by hot water extraction, anion-exchange and gel-permeation chromatography. Structure study shows FVP2 is an α-(1[rightward arrow]4)-d-glucan, with a single α-d-glucan at the C-6 position approximately every seven residues, along the main chain. The weight-average molecular weight is 1.89 x 10⁴ Da. Its biological activity was tested on hepatocytes. In vitro study indicates FVP2 can enhance the livability of primary culture of mouse hepatocytes and decrease the release of ALT as well as apoptosis of hepatocytes after CCl₄ intoxication.

Citation

Pang X, Yao W, Yang X, Xie C, Liu D, Zhang J, et al. (2007). Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium. Carbohydrate polymers https://doi.org/10.1016/j.carbpol.2007.04.010

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