Enoki, Winter Mushroom · 2012 · Journal Article
High relevancePurification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes
Flammulina velutipes
Key points
- A novel water-soluble heteropolysaccharide FVP60-B was extracted from the fruiting bodies of Flammulina velutipes with boiling water and purified by Sephacryl S-300 and S-400, which molecular weight was estimated to be 1.3×10⁴Da by HPLC. It is composed of l-fucose, d-mannose, d-glucose and d-galactose in a ratio of 1.16:0.82:1.00:3.08
- Sugar analysis, methylation analysis together with ¹H, ¹³C and 2D NMR spectroscopy disclosed that FVP60-B is consisted of a α-(1→6)-d-galactopyranan backbone with a terminal fucosyl, terminal glucosyl and α-(1→6)-d-mannopyranan units on O-2 of 2,6-O-substituted-d-galactosyl units
From the paper
Abstract
A novel water-soluble heteropolysaccharide FVP60-B was extracted from the fruiting bodies of Flammulina velutipes with boiling water and purified by Sephacryl S-300 and S-400, which molecular weight was estimated to be 1.3×10⁴Da by HPLC. It is composed of l-fucose, d-mannose, d-glucose and d-galactose in a ratio of 1.16:0.82:1.00:3.08. Sugar analysis, methylation analysis together with ¹H, ¹³C and 2D NMR spectroscopy disclosed that FVP60-B is consisted of a α-(1→6)-d-galactopyranan backbone with a terminal fucosyl, terminal glucosyl and α-(1→6)-d-mannopyranan units on O-2 of 2,6-O-substituted-d-galactosyl units.
Citation
Zhang Aq, Xiao Nn, Deng Yl, He Pf, Sun Pl (2012). Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes. Carbohydrate polymers https://doi.org/10.1016/j.carbpol.2011.10.061
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