Maitake, Hen of the Woods · 2012 · Research Support, Non U.S. Gov'T
Medium relevanceIsolation, purification and antioxidant activities of polysaccharides from Grifola frondosa.
Grifola frondosa
Key points
- The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order
- Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps
- Then the antioxidant activities of these three fractions were investigated in vitro
- The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong
- These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans
Metadata-grounded summary
Citation abstract
The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order. Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps. Then the antioxidant activities of these three fractions were investigated in vitro. The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong. These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans.
Citation
Chen GT, Ma XM, Liu ST, Liao YL, Zhao GQ (2012). Isolation, purification and antioxidant activities of polysaccharides from Grifola frondosa. Carbohydrate polymers https://doi.org/10.1016/j.carbpol.2012.02.045 PMID: 24750604
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