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Lion's Mane, Yamabushitake · 2014 · Research Support, Non U.S. Gov'T

Medium relevance

Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus.

Hericium erinaceus

Immune supportCognition & nerves
SpeciesLion's Mane, Yamabushitake
JournalCarbohydrate polymers
Year2014

Key points

  • The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied
  • In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions
  • The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03°C and a time of 33.79 min
  • The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE)
  • The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1
  • The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes

Metadata-grounded summary

Citation abstract

The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03°C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.

Citation

Zhu Y, Li Q, Mao G, Zou Y, Feng W, Zheng D, et al. (2014). Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus. Carbohydrate polymers https://doi.org/10.1016/j.carbpol.2013.09.099 PMID: 24299817

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