King Oyster, Eryngii · 2014 · Research Support, Non U.S. Gov'T
High relevanceThe excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36.
Pleurotus eryngii
Key points
- Previous study has verified the polysaccharide from the fruiting body of Pleurotus eryngii (PEPE) is capable of decreasing the lipid content in both of cell-line and mouse model
- However, little is known about underlying mechanisms and whether this bioactive polysaccharide exists in submerged culture
- Here, we verified the excreted polysaccharides EP and EP-1 from submersion culture of P. eryngii have the remarkable inhibitory effects on lipid accumulation in macrophage-derived foam cells
- Structure analysis indicates EP-1 consists of D-types of glucose, galactose and mannose with the main β(1 → 3)-glucan glycosidic linkage branched at O-6 by α-D-glucose while EP digested by β-1,3-glucanase fails to decrease the lipid accumulation, suggesting that the special structure is essential for its function
- Expression analysis suggests that EP is able to cause the down-regulation of the scavenger receptor-CD36 on both transcription and protein levels
- Most importantly, EP can be obtained by fermentation in a mass-production
Metadata-grounded summary
Citation abstract
Previous study has verified the polysaccharide from the fruiting body of Pleurotus eryngii (PEPE) is capable of decreasing the lipid content in both of cell-line and mouse model. However, little is known about underlying mechanisms and whether this bioactive polysaccharide exists in submerged culture. Here, we verified the excreted polysaccharides EP and EP-1 from submersion culture of P. eryngii have the remarkable inhibitory effects on lipid accumulation in macrophage-derived foam cells. Structure analysis indicates EP-1 consists of D-types of glucose, galactose and mannose with the main β(1 → 3)-glucan glycosidic linkage branched at O-6 by α-D-glucose while EP digested by β-1,3-glucanase fails to decrease the lipid accumulation, suggesting that the special structure is essential for its function. Expression analysis suggests that EP is able to cause the down-regulation of the scavenger receptor-CD36 on both transcription and protein levels. Most importantly, EP can be obtained by fermentation in a mass-production.
Citation
Chen J, Yong Y, Xia X, Wang Z, Liang Y, Zhang S, et al. (2014). The excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36. Carbohydrate polymers https://doi.org/10.1016/j.carbpol.2014.05.068 PMID: 25129711
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