Structure and chain conformation of a neutral polysaccharide from sclerotia of Polyporus umbellatus.

Polyporus umbellatus

Immune support

SpeciesZhu Ling, Umbrella Polypore

JournalCarbohydrate polymers

Year2017

Key points

A water-soluble heteropolysaccharide (PUP60W-1) was purified from the hot water extract of sclerotia of P. umbellatus by chromatography with DEAE Sepharose Fast Flow and Sephacryl S200 High-Resolution
The primary structure of PUP60W-1 was elucidated by GC, GC-MS and NMR. PUP60W-1 was identified as a highly branched polysaccharide composed of fucose, glucose and galactose in a ratio of 1.0:0.9:13.3
The main repeating unit was identified as α-(1→6)-d-galactopyranan backbone with substitution of terminal α-galactopyranosyl residues at O-2 for two out of every three main chain galactose residues
Its chain conformation was studied by atom force microscopy and size exclusion chromatography coupled with multiple detectors
The results revealed that PUP60W-1 had a molecular weight of 2.47×10 4 Da with a polydispersity index of 1.04, and existed in water as compact sphere structures which could be disrupted into smaller spherical chain blocks after dispersion with sodium dodecyl sulfate

Metadata-grounded summary

Citation abstract

A water-soluble heteropolysaccharide (PUP60W-1) was purified from the hot water extract of sclerotia of P. umbellatus by chromatography with DEAE Sepharose Fast Flow and Sephacryl S200 High-Resolution. The primary structure of PUP60W-1 was elucidated by GC, GC-MS and NMR. PUP60W-1 was identified as a highly branched polysaccharide composed of fucose, glucose and galactose in a ratio of 1.0:0.9:13.3. The main repeating unit was identified as α-(1→6)-d-galactopyranan backbone with substitution of terminal α-galactopyranosyl residues at O-2 for two out of every three main chain galactose residues. Its chain conformation was studied by atom force microscopy and size exclusion chromatography coupled with multiple detectors. The results revealed that PUP60W-1 had a molecular weight of 2.47×10 4 Da with a polydispersity index of 1.04, and existed in water as compact sphere structures which could be disrupted into smaller spherical chain blocks after dispersion with sodium dodecyl sulfate.

Citation

He PF, He L, Zhang AQ, Wang XL, Qu L, Sun PL (2017). Structure and chain conformation of a neutral polysaccharide from sclerotia of Polyporus umbellatus. Carbohydrate polymers https://doi.org/10.1016/j.carbpol.2016.08.041 PMID: 27702545

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