Back to search

Maitake, Hen of the Woods · 2009 · Research Support, Non U.S. Gov'T

Medium relevance

An unambiguous structural elucidation of a 1,3-beta-D-glucan obtained from liquid-cultured Grifola frondosa by solution NMR experiments.

Grifola frondosa

Immune support
SpeciesMaitake, Hen of the Woods
JournalCarbohydrate research
Year2009

Key points

  • Grifolan LE (GRN-LE), a purified beta-D-glucan, which is obtained from liquid-cultured Grifola frondosa, exhibits various biological activities, including antitumor effects
  • Significant progress has been made in the study of these effects
  • However, an unambiguous structural characterization of GRN-LE using NMR spectroscopy has not been carried out as yet
  • It is well accepted that the biological effects of a beta-glucan depend on its primary structure, conformation, and molecular weight
  • In the present study, we unambiguously elucidate the primary structure of GRN-LE using NMR spectroscopy
  • The data presented here reveal that GRN-LE comprises a 1,3-beta-D-glucan backbone with a single 1,6-beta-D-glucosyl side branching unit on every third residue

Metadata-grounded summary

Citation abstract

Grifolan LE (GRN-LE), a purified beta-D-glucan, which is obtained from liquid-cultured Grifola frondosa, exhibits various biological activities, including antitumor effects. Significant progress has been made in the study of these effects. However, an unambiguous structural characterization of GRN-LE using NMR spectroscopy has not been carried out as yet. It is well accepted that the biological effects of a beta-glucan depend on its primary structure, conformation, and molecular weight. In the present study, we unambiguously elucidate the primary structure of GRN-LE using NMR spectroscopy. The data presented here reveal that GRN-LE comprises a 1,3-beta-D-glucan backbone with a single 1,6-beta-D-glucosyl side branching unit on every third residue.

Citation

Tada R, Adachi Y, Ishibashi K, Ohno N (2009). An unambiguous structural elucidation of a 1,3-beta-D-glucan obtained from liquid-cultured Grifola frondosa by solution NMR experiments. Carbohydrate research https://doi.org/10.1016/j.carres.2008.11.005 PMID: 19084824

Open citation