Turkey Tail, Yun Zhi · 2007 · Research Support, Non U.S. Gov'T
Medium relevanceExoenzymes of Trametes versicolor can metabolize coplanar PCB congeners and hydroxy PCB.
Trametes versicolor
Key points
- Two fractions containing the oxidase activity toward 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) were obtained using ion-exchange DEAE-Sepharose column chromatography of the culture fluid of white-rot fungus, Trametes versicolor
- These two fractions can reduce the level of coplanar PCB congeners (Co-PCBs)
- The ABTS oxidase in the first fraction passed through the DEAE-Sepharose column
- The ABTS oxidase in the second fraction was adsorbed to the column at <pH 5.6
- Overall, the first fraction has a higher activity to reduce each level of Co-PCBs than the second fraction does
- In equally chlorinated congeners, non-ortho congeners were less metabolized than ortho congeners; a 4-hydroxy-pentachlorobiphenyl (hydroxy PeCB) was more susceptible to metabolism than a structurally related non-hydroxy PeCB or HxCB
Metadata-grounded summary
Citation abstract
Two fractions containing the oxidase activity toward 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) were obtained using ion-exchange DEAE-Sepharose column chromatography of the culture fluid of white-rot fungus, Trametes versicolor. These two fractions can reduce the level of coplanar PCB congeners (Co-PCBs). The ABTS oxidase in the first fraction passed through the DEAE-Sepharose column. The ABTS oxidase in the second fraction was adsorbed to the column at <pH 5.6. Overall, the first fraction has a higher activity to reduce each level of Co-PCBs than the second fraction does. In equally chlorinated congeners, non-ortho congeners were less metabolized than ortho congeners; a 4-hydroxy-pentachlorobiphenyl (hydroxy PeCB) was more susceptible to metabolism than a structurally related non-hydroxy PeCB or HxCB.
Citation
Takagi S, Shirota C, Sakaguchi K, Suzuki J, Sue T, Nagasaka H, et al. (2007). Exoenzymes of Trametes versicolor can metabolize coplanar PCB congeners and hydroxy PCB. Chemosphere https://doi.org/10.1016/j.chemosphere.2006.05.090 PMID: 17250871
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