Laccases from Aureobasidium pullulans.

Trametes versicolor

OncologyImmune support

SpeciesTurkey Tail, Yun Zhi

JournalEnzyme and microbial technology

Year2013

Key points

Laccases are polyphenol oxidases (EC 1.10.3.2) that have numerous industrial and bioremediation applications
In this study, 41 strains of the fungus Aureobasidium pullulans were examined for laccase production
Enzymes from A. pullulans were distinct from those from lignin-degrading fungi and associated with pigment production
Laccases from strains in phylogenetic clade 5, which produced a dark vinaceous pigment, exhibited a temperature optimum of 50-60°C and were stable for an hour at 50°C, unlike enzymes from the lignin-degrading fungi Trametes versicolor and Pycnoporus cinnabarinus
Laccase purified from A. pullulans strain NRRL 50381, a representative of clade 5, was glycosylated but had a molecular weight of 60-70kDa after Endo H treatment
Laccase purified from strain NRRL Y-2568, which produced a dark olivaceous pigment, was also glycosylated, but had a molecular weight of greater than 100kDa after Endo H treatment

Metadata-grounded summary

Citation abstract

Laccases are polyphenol oxidases (EC 1.10.3.2) that have numerous industrial and bioremediation applications. Laccases are well known as lignin-degrading enzymes, but these enzymes can play numerous other roles in fungi. In this study, 41 strains of the fungus Aureobasidium pullulans were examined for laccase production. Enzymes from A. pullulans were distinct from those from lignin-degrading fungi and associated with pigment production. Laccases from strains in phylogenetic clade 5, which produced a dark vinaceous pigment, exhibited a temperature optimum of 50-60°C and were stable for an hour at 50°C, unlike enzymes from the lignin-degrading fungi Trametes versicolor and Pycnoporus cinnabarinus. Laccase purified from A. pullulans strain NRRL 50381, a representative of clade 5, was glycosylated but had a molecular weight of 60-70kDa after Endo H treatment. Laccase purified from strain NRRL Y-2568, which produced a dark olivaceous pigment, was also glycosylated, but had a molecular weight of greater than 100kDa after Endo H treatment.

Citation

Rich JO, Leathers TD, Anderson AM, Bischoff KM, Manitchotpisit P (2013). Laccases from Aureobasidium pullulans. Enzyme and microbial technology https://doi.org/10.1016/j.enzmictec.2013.03.015 PMID: 23683702

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