Metabolic remodeling during fructification of enoki mushroom.

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Citation abstract

This study investigated the patterns of change and differences in metabolites between white and golden enoki (Flammulina velutipes) mycelium and across stages of fruit-body growth of golden enoki, which was cultured under controlled conditions. Metabolic differences were observed between the mycelium, juvenile and aged caps, and the stipe, which highlight stage-specific shifts in metabolite concentrations. Nucleotides had significantly lower concentrations in mycelium and displayed progressively smaller fold changes as the mushroom matured. These nucleotides could be valuable as biomarkers for fruit-body formation and early development. Glycine, homocitrulline, lysine, ornithine, urea, and proline were predominantly present in the mycelium and have potential for use in food supplementation due to their documented health benefits. Of particular interest is ornithine, which was the sole metabolite that was downregulated in the cap and has potential as a biomarker for cap-stipe differentiation.