Enhancing the degradation of Aflatoxin B1 by co-cultivation of two fungi strains with the improved production of detoxifying enzymes.

Pleurotus ostreatus

Metabolic health

SpeciesOyster Mushroom

JournalFood chemistry

Year2022

Key points

After the co-culture of Aspergillus niger and Pleurotus ostreatus, the obtained extracellular crude enzymes solution was employed to aflatoxin B 1 (AFB 1 ) degradation
The maximum AFB 1 degradation with co-culture reached 93.4%, which increased by 65.9% and 37.6%, respectively, compared with those of the mono-culture of Pleurotus ostreatus and Aspergillus niger
The molecular weight of the key detoxifying enzymes isolated by ultrafiltration was 58 and 63 kDa by SDS-PAGE analysis
The purified detoxifying enzymes had a high detoxification effect on AFB 1 with the degradation rate of 94.7%
It was found that the co-culture of Pleurotus ostreatus and Aspergillus niger promoted the production of 58 and 63 kDa detoxifying enzymes to enhance the AFB 1 degradation
The chemical structure of major degradation products of AFB 1 by the mixed cultures were preliminarily identified by LC-Triple TOF MS. Two pathways of AFB 1 degradation were inferred with the high potential of fungal co-cultivations for AFB 1 detoxification applications

Metadata-grounded summary

Citation abstract

After the co-culture of Aspergillus niger and Pleurotus ostreatus, the obtained extracellular crude enzymes solution was employed to aflatoxin B 1 (AFB 1 ) degradation. The maximum AFB 1 degradation with co-culture reached 93.4%, which increased by 65.9% and 37.6%, respectively, compared with those of the mono-culture of Pleurotus ostreatus and Aspergillus niger. The molecular weight of the key detoxifying enzymes isolated by ultrafiltration was 58 and 63 kDa by SDS-PAGE analysis. The purified detoxifying enzymes had a high detoxification effect on AFB 1 with the degradation rate of 94.7%. It was found that the co-culture of Pleurotus ostreatus and Aspergillus niger promoted the production of 58 and 63 kDa detoxifying enzymes to enhance the AFB 1 degradation. The chemical structure of major degradation products of AFB 1 by the mixed cultures were preliminarily identified by LC-Triple TOF MS. Two pathways of AFB 1 degradation were inferred with the high potential of fungal co-cultivations for AFB 1 detoxification applications.

Citation

Wang L, Huang W, Shen Y, Zhao Y, Wu D, Yin H, et al. (2022). Enhancing the degradation of Aflatoxin B1 by co-cultivation of two fungi strains with the improved production of detoxifying enzymes. Food chemistry https://doi.org/10.1016/j.foodchem.2021.131092 PMID: 34543924

Open citation