Detoxification of ochratoxin A and zearalenone by Pleurotus ostreatus during in vitro gastrointestinal digestion.

Pleurotus ostreatus

Metabolic healthGut & microbiomeLiver support

SpeciesOyster Mushroom

JournalFood chemistry

Year2022

Key points

Powdered Pleurotusostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal
Ochratoxin α was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, whereas ZEN was adsorbed by PO. OTA was totally eliminated at pH 5 and 7, while ZEN was better adsorbed at pH 3
In simulated gastrointestinal conditions conducted without feed matrices, PO eliminated OTA by 85% and ZEN by 23% at the end of the intestinal phase
When digestions were conducted with ground feed and cornmeal, PO eliminated OTA by 13 and 34%, and ZEN by 20 and 2%, respectively
PO demonstrated great potential for OTA detoxification, but the feed matrices adsorbed the mycotoxins, reducing their bioaccessibility considerably in the oral and gastric phases and negatively influencing PO detoxification capacity

Metadata-grounded summary

Citation abstract

Powdered Pleurotusostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal. Ochratoxin α was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, whereas ZEN was adsorbed by PO. OTA was totally eliminated at pH 5 and 7, while ZEN was better adsorbed at pH 3. In simulated gastrointestinal conditions conducted without feed matrices, PO eliminated OTA by 85% and ZEN by 23% at the end of the intestinal phase. When digestions were conducted with ground feed and cornmeal, PO eliminated OTA by 13 and 34%, and ZEN by 20 and 2%, respectively. PO demonstrated great potential for OTA detoxification, but the feed matrices adsorbed the mycotoxins, reducing their bioaccessibility considerably in the oral and gastric phases and negatively influencing PO detoxification capacity.

Citation

Nobre C, González A, Losoya C, Teixeira JA, Belmares R, Abrunhosa L (2022). Detoxification of ochratoxin A and zearalenone by Pleurotus ostreatus during in vitro gastrointestinal digestion. Food chemistry https://doi.org/10.1016/j.foodchem.2022.132525 PMID: 35227998

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