Possible role of Tremella fuciformis polysaccharide in modulating in vitro digestion of potato starch digesta.

Tremella fuciformis

Immune supportGut & microbiomeSkin & hydration

SpeciesSnow Fungus, Silver Ear

JournalFood chemistry: X

Year2025

Key points

The effect of deproteinized Tremella olysaccharide (DTP) on the in vitro digestion of potato starch (PS) was investigated
DTP reduced the hydrolysis rate of PS in a concentration-dependent manner, with digestion rates of 76.99 %, 72.03 %, and 29.73 % after 180 min for 0.3, 0.6, and 1.2 mg/mL DTP, respectively
DTP delayed digestion by shifting the kinetics from single-phase to biphasic behavior
It significantly inhibited pancreatic α-amylase (IC 50 = 5.76 mg/mL) by modifying the enzyme's secondary structure and reducing substrate binding
Additionally, DTP enhanced digesta viscosity, which may have contributed to the reduction in postprandial glucose release, thereby supporting its role as a potential hypoglycemic agent
However, DTP exhibited minimal glucose adsorption, binding only 1.2 % of 100 mM glucose at 1.2 mg/mL. These findings suggest that DTP reduces starch digestion primarily by inhibiting α-amylase activity and increasing digestive viscosity, providing insights into its hypoglycemic potential

Metadata-grounded summary

Citation abstract

The effect of deproteinized Tremella olysaccharide (DTP) on the in vitro digestion of potato starch (PS) was investigated. DTP reduced the hydrolysis rate of PS in a concentration-dependent manner, with digestion rates of 76.99 %, 72.03 %, and 29.73 % after 180 min for 0.3, 0.6, and 1.2 mg/mL DTP, respectively. DTP delayed digestion by shifting the kinetics from single-phase to biphasic behavior. It significantly inhibited pancreatic α-amylase (IC 50 = 5.76 mg/mL) by modifying the enzyme's secondary structure and reducing substrate binding. Additionally, DTP enhanced digesta viscosity, which may have contributed to the reduction in postprandial glucose release, thereby supporting its role as a potential hypoglycemic agent. However, DTP exhibited minimal glucose adsorption, binding only 1.2 % of 100 mM glucose at 1.2 mg/mL. These findings suggest that DTP reduces starch digestion primarily by inhibiting α-amylase activity and increasing digestive viscosity, providing insights into its hypoglycemic potential.

Citation

Zhang X, Fu Y, Wang H, Shen Q, Liu Z, Chi Y (2025). Possible role of Tremella fuciformis polysaccharide in modulating in vitro digestion of potato starch digesta. Food chemistry: X https://doi.org/10.1016/j.fochx.2025.102759 PMID: 40704183

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