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Nameko, Nametake · 2015 · Research Support, Non U.S. Gov'T

Medium relevance

Effects of Pholiota nameko polysaccharide on NF-κB pathway of murine bone marrow-derived dendritic cells.

Pholiota microspora

Immune support
SpeciesNameko, Nametake
JournalInternational journal of biological macromolecules
Year2015

Key points

  • This study investigated the effect of a polysaccharide purified from Pholiota nameko (PNPS-1) on the NF-κB signaling pathway of murine bone marrow-derived dendritic cells (BMDCs) and relevant mechanisms
  • The results showed that PNPS-1 could decrease the expression of maturation markers CD40 and CD80 on BMDCs
  • PNPS-1 also could decrease the mRNA expression of Myd88, TRAF6, TIRAP, IRAKI, IKBKB, NFKB1, NFKB2 and RelA in immature BMDCs determined by RT-PCR, and decreased the IKKβ and P65 production in BMDCs determined by Western blot, and decreased the NF-кB P65 production determined by ELISA. In addition, the effects of PNPS-1 on BMDCs were significantly impaired by treating the cells with anti-TLR2 antibody prior to PNPS-1 treatment, implying direct interaction between PNPS-1 and TLR2 on cell surface
  • These results indicate that PNPS-1 regulates BMDCs through TLR2 and downstream NF-кB signalings

Metadata-grounded summary

Citation abstract

This study investigated the effect of a polysaccharide purified from Pholiota nameko (PNPS-1) on the NF-κB signaling pathway of murine bone marrow-derived dendritic cells (BMDCs) and relevant mechanisms. The results showed that PNPS-1 could decrease the expression of maturation markers CD40 and CD80 on BMDCs. PNPS-1 also could decrease the mRNA expression of Myd88, TRAF6, TIRAP, IRAKI, IKBKB, NFKB1, NFKB2 and RelA in immature BMDCs determined by RT-PCR, and decreased the IKKβ and P65 production in BMDCs determined by Western blot, and decreased the NF-кB P65 production determined by ELISA. In addition, the effects of PNPS-1 on BMDCs were significantly impaired by treating the cells with anti-TLR2 antibody prior to PNPS-1 treatment, implying direct interaction between PNPS-1 and TLR2 on cell surface. These results indicate that PNPS-1 regulates BMDCs through TLR2 and downstream NF-кB signalings.

Citation

Li H, Tao Y, Zhao P, Huai L, Zhi D, Liu J, et al. (2015). Effects of Pholiota nameko polysaccharide on NF-κB pathway of murine bone marrow-derived dendritic cells. International journal of biological macromolecules https://doi.org/10.1016/j.ijbiomac.2015.03.011 PMID: 25812973

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