Zhu Ling, Umbrella Polypore · 2016 · Research Support, Non U.S. Gov'T
High relevanceStructure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
Polyporus umbellatus
Key points
- A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography
- PUP60S2 was a polysaccharide comprised of about 22.3% glucuronic acid
- Monosaccharide composition analysis showed that PUP60S2 was only comprised of glucose and glucuronic acid
- Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units
- The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner
Metadata-grounded summary
Citation abstract
A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography. PUP60S2 was a polysaccharide comprised of about 22.3% glucuronic acid. Monosaccharide composition analysis showed that PUP60S2 was only comprised of glucose and glucuronic acid. Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units. The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
Citation
He PF, Zhang AQ, Wang XL, Qu L, Li GL, Li YP, et al. (2016). Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia. International journal of biological macromolecules https://doi.org/10.1016/j.ijbiomac.2015.10.032 PMID: 26476244
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