Green magnetic hydrogels synthesis, characterization and flavourzyme immobilization based on chitin from Hericium erinaceus residue and polyvinyl alcohol.

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Citation abstract

In this study, a novel magnetic sensitive hydrogel was prepared based on fungal chitin extracted from Hericium erinaceus residue and polyvinyl alcohol (PVA) through a green and facile procedure of repeated freezing-thaw and in situ decoration of Fe 3 O 4. The prepared hydrogels were characterized and applied for flavourzyme immobilization. The results showed that magnetic Fe 3 O 4 was successfully introduced into chitin/PVA matrix due to the porous networks of the hydrogels, and the prepared hydrogels displayed superparamagnetic behavior and improved gel strength due to the presence of Fe 3 O 4. The efficiency for flavourzyme immobilization was influenced by the mass ratios of chitin/PVA. The optimal flavourzyme immobilization carrier was obtained at 1:2 ratio of chitin/PVA. Compared with free flavourzyme, the hydrogels-embedded-flavourzyme (HEF) showed uniform optimal reaction temperature (50 °C), but slightly shifted pH value at 6.0. More importantly, HEF displayed preferable thermal stability and great reusability. This work provided a relatively simple, facile, low-cost and eco-friendly way to fabricate magnetic hydrogels, and the prepared hydrogels could be served as the promising material for enzyme immobilization application.