Maitake, Hen of the Woods · 2020 · Journal Article
Medium relevancePurification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body.
Grifola frondosa
Key points
- The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order
- Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps
- Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR)
- GC-MS and methylation analysis that linkages were mainly β-1,3 and β-1,6, β-1,4 and β-1,2 bonds in WIP. Seven main oligomer products were detected and their structures characterized by mass spectrum
- Experimental results shown the similarity in structure between water soluble polysaccharides (WSP) and water insoluble polysaccharides (WIP), thus WSP can be the product of cell wall by breakdown
Metadata-grounded summary
Citation abstract
The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). GC-MS and methylation analysis that linkages were mainly β-1,3 and β-1,6, β-1,4 and β-1,2 bonds in WIP. Seven main oligomer products were detected and their structures characterized by mass spectrum. Experimental results shown the similarity in structure between water soluble polysaccharides (WSP) and water insoluble polysaccharides (WIP), thus WSP can be the product of cell wall by breakdown.
Citation
Yiasmin MN, Islam MS, He H, Liu Y, Wang M, Yang R, et al. (2020). Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body. International journal of biological macromolecules https://doi.org/10.1016/j.ijbiomac.2020.08.037 PMID: 32791276
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