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Turkey Tail, Yun Zhi · 2015 · Research Support, Non U.S. Gov'T

Low relevance

Immobilization of laccase in a sponge-like hydrogel for enhanced durability in enzymatic degradation of dye pollutants.

Trametes versicolor

OncologyImmune support
SpeciesTurkey Tail, Yun Zhi
JournalJournal of colloid and interface science
Year2015

Key points

  • Scanning electron microscopy and nitrogen adsorption-desorption tests demonstrated that channels of diameter of 10-20 μm were regularly distributed throughout the sponge-like Lac-PAM-CTS. Besides, there were massive mesopores and macropores in the lamellar walls of the hydrogel
  • Such a network structure reduced the diffusion resistance of the hydrogel to the target substrates
  • As compared to free laccase, the Lac-PAM-CTS showed enhanced thermal and chemical stability
  • The positive surface charge of the Lac-PAM-CTS endowed it with a pre-enrichment effect in the treatment of anionic dyes
  • In a continuous six-cycle batch decoloration of Malachite Green, the Lac-PAM-CTS showed much better durability than the free laccase
  • The results here suggest that sponge-like hydrogel is a good supporting matrix for laccase

Metadata-grounded summary

Citation abstract

A highly stable and efficient biocatalyst was fabricated by encapsulating Trametes versicolor laccase within a chitosan grafted polyacrylamide hydrogel (denoted as Lac-PAM-CTS). Scanning electron microscopy and nitrogen adsorption-desorption tests demonstrated that channels of diameter of 10-20 μm were regularly distributed throughout the sponge-like Lac-PAM-CTS. Besides, there were massive mesopores and macropores in the lamellar walls of the hydrogel. Such a network structure reduced the diffusion resistance of the hydrogel to the target substrates. The recovered activity of the obtained Lac-PAM-CTS was 40.8%. As compared to free laccase, the Lac-PAM-CTS showed enhanced thermal and chemical stability. The positive surface charge of the Lac-PAM-CTS endowed it with a pre-enrichment effect in the treatment of anionic dyes. In a continuous six-cycle batch decoloration of Malachite Green, the Lac-PAM-CTS showed much better durability than the free laccase. The results here suggest that sponge-like hydrogel is a good supporting matrix for laccase.

Citation

Sun H, Yang H, Huang W, Zhang S (2015). Immobilization of laccase in a sponge-like hydrogel for enhanced durability in enzymatic degradation of dye pollutants. Journal of colloid and interface science https://doi.org/10.1016/j.jcis.2015.03.037 PMID: 25841061

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