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Enoki, Winter Mushroom · 2018 · Evaluation Study

Medium relevance

Application of thermal stability difference to remove flammutoxin in fungal immunomodulatory protein, FIP-fve, extract from Flammulina velutipes.

Flammulina velutipes

Immune support
SpeciesEnoki, Winter Mushroom
JournalJournal of food and drug analysis
Year2018

Key points

  • Fungal immunomodulatory protein (FIP-fve) is a potential functional food ingredient
  • However, undesirable component flammutoxin (FTX) would occur in the extracted fraction of FIP-fve
  • In this paper, an application of heating processing instead of the intensive separation process was employed in fractionation of FIP-fve, meanwhile, exclusion of FTX was reached
  • Cold water could effectively extract FIP-fve and FTX from fresh mushroom without acetic acid and disulfide-bond breaking agent β-mercaptoethanol commonly used in biochemical studies
  • Heating cold water extract contained 580 μg/mL FIP-fve and 452 μg/mL FTX at 60 °C for 5 min could effectively exclude FTX and remain 75% of FIP-fve
  • Adding 0.1 M trehalose or 20% ethanol did not significantly alter the stability of both proteins

Metadata-grounded summary

Citation abstract

Fungal immunomodulatory protein (FIP-fve) is a potential functional food ingredient. However, undesirable component flammutoxin (FTX) would occur in the extracted fraction of FIP-fve. In this paper, an application of heating processing instead of the intensive separation process was employed in fractionation of FIP-fve, meanwhile, exclusion of FTX was reached. Contents of FIP-fve and FTX were monitored by HPLC-UV-ESI-MS. Both FIP-fve and FTX had higher thermal stability in a lower concentration solution. Cold water could effectively extract FIP-fve and FTX from fresh mushroom without acetic acid and disulfide-bond breaking agent β-mercaptoethanol commonly used in biochemical studies. Heating cold water extract contained 580 μg/mL FIP-fve and 452 μg/mL FTX at 60 °C for 5 min could effectively exclude FTX and remain 75% of FIP-fve. Adding 0.1 M trehalose or 20% ethanol did not significantly alter the stability of both proteins. The method developed is an applicable procedure for preparing FIP-fve solution free of FTX.

Citation

Tung CH, Lin CC, Wang HJ, Chen SF, Sheu F, Lu TJ (2018). Application of thermal stability difference to remove flammutoxin in fungal immunomodulatory protein, FIP-fve, extract from Flammulina velutipes. Journal of food and drug analysis https://doi.org/10.1016/j.jfda.2017.12.010 PMID: 29976393

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