Cordyceps, Scarlet Club · 2015 · Research Support, Non U.S. Gov'T
High relevanceQuantitative real-time PCR (qPCR)--based tool for detection and quantification of Cordyceps militaris in soil.
Cordyceps militaris
Key points
- A quantitative real-time PCR using a primer pair CM2946F/CM3160R was developed for specific detection and quantification of Cordyceps militaris from soil
- Standard curves were obtained for genomic DNA and DNA extracts from autoclaved soil with a certain dose of C. militaris suspension
- C. militaris was detected from two forest soil samples out of ten that were collected when fruit bodies of C. militaris were found
- This method seemed effective in detection of C. militaris in the soil and useful for rapid and reliable quantification of C. militaris in different ecosystems
Metadata-grounded summary
Citation abstract
A quantitative real-time PCR using a primer pair CM2946F/CM3160R was developed for specific detection and quantification of Cordyceps militaris from soil. Standard curves were obtained for genomic DNA and DNA extracts from autoclaved soil with a certain dose of C. militaris suspension. C. militaris was detected from two forest soil samples out of ten that were collected when fruit bodies of C. militaris were found. This method seemed effective in detection of C. militaris in the soil and useful for rapid and reliable quantification of C. militaris in different ecosystems.
Citation
Saragih SA, Takemoto S, Hisamoto Y, Fujii M, Sato H, Kamata N (2015). Quantitative real-time PCR (qPCR)--based tool for detection and quantification of Cordyceps militaris in soil. Journal of invertebrate pathology https://doi.org/10.1016/j.jip.2014.11.002 PMID: 25446034
Open citation