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King Oyster, Eryngii · 2018 · Research Support, Non U.S. Gov'T

High relevance

Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene.

Pleurotus eryngii

Gut & microbiome
SpeciesKing Oyster, Eryngii
JournalJournal of microbiological methods
Year2018

Key points

  • We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts
  • The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdi1 gene is an efficient selective marker

Metadata-grounded summary

Citation abstract

We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdi1 gene is an efficient selective marker.

Citation

Shang J, Li Y, Yang R, Wang Y, Mao W, Tang L, et al. (2018). Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene. Journal of microbiological methods https://doi.org/10.1016/j.mimet.2018.07.006 PMID: 30017848

Open citation