Establishment of a CRISPR/Cas9 protocol by biolistic transformation of the filamentous basidiomycete Pleurotus ostreatus.

Pleurotus ostreatus

Metabolic health

SpeciesOyster Mushroom

JournalJournal of microbiological methods

Year2026

Key points

Genome editing was successfully performed by introducing a CRISPR/Cas9 expression vector through biolistic transformation of Pleurotus ostreatus, an oyster mushroom
This method can be applied for genome editing in filamentous basidiomycetes without protoplasting
To our knowledge, this is the first report of genome editing with biolistic transformation in filamentous basidiomycetes

Metadata-grounded summary

Citation abstract

Genome editing was successfully performed by introducing a CRISPR/Cas9 expression vector through biolistic transformation of Pleurotus ostreatus, an oyster mushroom. This method can be applied for genome editing in filamentous basidiomycetes without protoplasting. To our knowledge, this is the first report of genome editing with biolistic transformation in filamentous basidiomycetes.

Citation

Terakawa Y, Koshi D, Kawauchi M, Nakazawa T, An GH, Honda Y (2026). Establishment of a CRISPR/Cas9 protocol by biolistic transformation of the filamentous basidiomycete Pleurotus ostreatus. Journal of microbiological methods https://doi.org/10.1016/j.mimet.2026.107430 PMID: 41672309

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