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King Oyster, Eryngii · 2020 · Research Support, Non U.S. Gov'T

Medium relevance

Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus.

Pleurotus eryngii

SpeciesKing Oyster, Eryngii
JournalJournal of microbiological methods
Year2020

Key points

  • Using the carboxin resistance gene from Pleurotus eryngii as a selective marker, we introduced foreign DNA into the arthroconidia of Hypsizygus marmoreus through Agrobacterium-mediated transformation
  • The function of the exogenous GUS (β-glucuronidase) gene driven by the CaMV35S promoter was detected in the transformants

From the paper

Abstract

Using the carboxin resistance gene from Pleurotus eryngii as a selective marker, we introduced foreign DNA into the arthroconidia of Hypsizygus marmoreus through Agrobacterium-mediated transformation. The function of the exogenous GUS (β-glucuronidase) gene driven by the CaMV35S promoter was detected in the transformants.

Citation

Bao D, Huang Z, Li Y, Zhou C, Li Y, Wan J, et al. (2020). Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus. Journal of microbiological methods https://doi.org/10.1016/j.mimet.2020.105878 PMID: 32092329

Open citation