A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea.

Pleurotus ostreatus

Metabolic health

SpeciesOyster Mushroom

JournalJournal of microbiological methods

Year2020

Key points

A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique
It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation experiments

Metadata-grounded summary

Citation abstract

A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation experiments.

Citation

Nguyen DX, Nakazawa T, Myo G, Inoue C, Kawauchi M, Sakamoto M, et al. (2020). A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea. Journal of microbiological methods https://doi.org/10.1016/j.mimet.2020.106053 PMID: 32918936

Open citation