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Enoki, Winter Mushroom · 2010 · Research Support, Non U.S. Gov'T

High relevance

Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes.

Flammulina velutipes

Immune support
SpeciesEnoki, Winter Mushroom
JournalMicrobiological research
Year2010

Key points

  • An electrophoretic karyotype of Korean Flammulina velutipes was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis
  • The monokaryotic progenies (4019-20 and 4019-18) and a dikaryotic strain (4019-2018) had 6-8 chromosomes, with sizes ranging from 1.6 to 5.8 megabase (Mb) pairs
  • Among the 3 strains that were examined, strain 4019-20 resolved into at least 7 chromosomes, with a total genome size of approximately 26.7Mb
  • We selected several chromosome-specific genes from the cDNA library of F. velutipes using Southern hybridization analysis
  • In order to determine the whole genome sequence, we constructed a bacterial artificial chromosome (BAC) library of the 4019-20 strain
  • The BAC library comprised 3840 clones, and the insert size ranged from 60 to 228kb, with an average size of 136kb

Metadata-grounded summary

Citation abstract

An electrophoretic karyotype of Korean Flammulina velutipes was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The monokaryotic progenies (4019-20 and 4019-18) and a dikaryotic strain (4019-2018) had 6-8 chromosomes, with sizes ranging from 1.6 to 5.8 megabase (Mb) pairs. Among the 3 strains that were examined, strain 4019-20 resolved into at least 7 chromosomes, with a total genome size of approximately 26.7Mb. We selected several chromosome-specific genes from the cDNA library of F. velutipes using Southern hybridization analysis. In order to determine the whole genome sequence, we constructed a bacterial artificial chromosome (BAC) library of the 4019-20 strain. The BAC library comprised 3840 clones, and the insert size ranged from 60 to 228kb, with an average size of 136kb.

Citation

Park YJ, Kim JK, Kong WS, Song ES, Lee CS, Kim H, et al. (2010). Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes. Microbiological research https://doi.org/10.1016/j.micres.2009.06.003 PMID: 19720512

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