Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis

Ophiocordyceps sinensis

Cognition & nervesEnergy & fatigue

SpeciesCordyceps, Caterpillar Fungus

JournalMycoscience

Year2014

Key points

Light plays an important role during both sexual and asexual growth of Ophiocordyceps sinensis, one of the best-known traditional Chinese medicines
In the present study, we cloned Oswc-1, the homologue of the blue light photoreceptor Ncwc-1 of Neurospora crassa, from O. sinensis by Hi-tail polymerase chain reaction
The deduced amino acid sequence of Oswc-1 contains the similar function domains as NcWC-1 including transcriptional activation, LOV (Light, Oxygen, or Voltage), PAS (Per-Arnt-Sim) and Zinc Finger domains
Phylogenetic analysis based on fungal WC-1 supported OsWC-1 was a blue light receptor
The expression of Oswc-1 messenger RNA was up-regulated upon irradiation significantly

From the paper

Abstract

Light plays an important role during both sexual and asexual growth of Ophiocordyceps sinensis, one of the best-known traditional Chinese medicines. In the present study, we cloned Oswc-1, the homologue of the blue light photoreceptor Ncwc-1 of Neurospora crassa, from O. sinensis by Hi-tail polymerase chain reaction. The deduced amino acid sequence of Oswc-1 contains the similar function domains as NcWC-1 including transcriptional activation, LOV (Light, Oxygen, or Voltage), PAS (Per-Arnt-Sim) and Zinc Finger domains. Phylogenetic analysis based on fungal WC-1 supported OsWC-1 was a blue light receptor. The expression of Oswc-1 messenger RNA was up-regulated upon irradiation significantly.

Citation

Tao Yang Weiping Xiong Caihong Dong (2014). Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis. Mycoscience https://doi.org/10.1016/j.myc.2013.09.003

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