Expression of tyrosinase genes associated with fruiting body formation and pigmentation in Grifola frondosa

Grifola frondosa

Immune supportSkin & hydration

SpeciesMaitake, Hen of the Woods

JournalMycoscience

Year2019

Key points

Chemical and physical properties (including ultraviolet spectrum) of a brown pigment isolated from Grifola frondosa fruiting bodies were almost identical to those of fungal melanin
From the full genome sequence of G. frondosa, genes involved in the melanin biosynthesis pathway of this polypore mushroom were identified, including tyrosinases (tyr1 and tyr2)
Transcriptional analyses showed dramatic changes only in the expression of tyr2 in the primordial stage
Immuno-electron microscopy using anti-tyrosinase antibodies showed that TYR2 localizes in the cell walls of primordia
Therefore, TYR2 may be closely associated with melanin biosynthesis in this polypore mushroom G. frondosa, and melanin might be produced in the cell wall

From the paper

Abstract

Chemical and physical properties (including ultraviolet spectrum) of a brown pigment isolated from Grifola frondosa fruiting bodies were almost identical to those of fungal melanin. From the full genome sequence of G. frondosa, genes involved in the melanin biosynthesis pathway of this polypore mushroom were identified, including tyrosinases (tyr1 and tyr2). Transcriptional analyses showed dramatic changes only in the expression of tyr2 in the primordial stage. Immuno-electron microscopy using anti-tyrosinase antibodies showed that TYR2 localizes in the cell walls of primordia. Therefore, TYR2 may be closely associated with melanin biosynthesis in this polypore mushroom G. frondosa, and melanin might be produced in the cell wall.

Citation

Kawaguchi N, Hayashi M, Nakano S, Shimomura N, Yamaguchi T, Aimi T (2019). Expression of tyrosinase genes associated with fruiting body formation and pigmentation in Grifola frondosa. Mycoscience https://doi.org/10.1016/j.myc.2019.04.003

Open citation