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King Oyster, Eryngii · 2000 · Research Support, Non U.S. Gov'T

Low relevance

Southern blot screening for lignin peroxidase and aryl-alcohol oxidase genes in 30 fungal species.

Pleurotus eryngii

Gut & microbiome
SpeciesKing Oyster, Eryngii
JournalJournal of biotechnology
Year2000

Key points

  • Screening to detect genes encoding lignin peroxidase (LiP) and aryl-alcohol oxidase (AAO) has been carried out with 30 fungal strain using DNA probes from genes lpo of Phanerochaete chrysosporium (encoding LiP isoenzyme H8) and aao of Pleurotus eryngii
  • Evidence for the presence of genes closely related to lpo was found in Bjerkandera adusta, Fomes fomentarius, Ganoderma applanatum, Ganoderma australe, Lentinula degener, Peniophora gigantea, P. chrysosporium, Phanerochaete flavido-alba and Trametes tersicolor, whereas the gene aao was detected in Pleurotus species and B. adusta
  • The presence of both genes was only detected in B. adusta
  • These results suggest that different enzymatic system, formed by enzymes encoded by different genes, are responsible for lignin degradation by white-rot fungi

Metadata-grounded summary

Citation abstract

Screening to detect genes encoding lignin peroxidase (LiP) and aryl-alcohol oxidase (AAO) has been carried out with 30 fungal strain using DNA probes from genes lpo of Phanerochaete chrysosporium (encoding LiP isoenzyme H8) and aao of Pleurotus eryngii. Evidence for the presence of genes closely related to lpo was found in Bjerkandera adusta, Fomes fomentarius, Ganoderma applanatum, Ganoderma australe, Lentinula degener, Peniophora gigantea, P. chrysosporium, Phanerochaete flavido-alba and Trametes tersicolor, whereas the gene aao was detected in Pleurotus species and B. adusta. The presence of both genes was only detected in B. adusta. These results suggest that different enzymatic system, formed by enzymes encoded by different genes, are responsible for lignin degradation by white-rot fungi.

Citation

Varela E, Martínez AT, Martínez MJ (2000). Southern blot screening for lignin peroxidase and aryl-alcohol oxidase genes in 30 fungal species. Journal of biotechnology https://doi.org/10.1016/s0168-1656(00)00323-0 PMID: 11051421

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