Back to search

Enoki, Winter Mushroom · 2009 · Research Support, Non U.S. Gov'T

Low relevance

FIP-fve stimulates interferon-gamma production via modulation of calcium release and PKC-alpha activation.

Flammulina velutipes

Immune support
SpeciesEnoki, Winter Mushroom
JournalJournal of agricultural and food chemistry
Year2009

Key points

  • Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells
  • However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood
  • Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration
  • ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976
  • In conclusion, Ca2+ release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs

Metadata-grounded summary

Citation abstract

Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells. However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood. Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration. ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976. In conclusion, Ca2+ release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs.

Citation

Ou CC, Hsiao YM, Wu WJ, Tasy GJ, Ko JL, Lin MY (2009). FIP-fve stimulates interferon-gamma production via modulation of calcium release and PKC-alpha activation. Journal of agricultural and food chemistry https://doi.org/10.1021/jf902725s PMID: 19919129

Open citation