Wood Ear, Kikurage · 2004 · Research Support, Non U.S. Gov'T
Medium relevanceProduction of an anti-complement exo-polymer produced by Auricularia auricula-judae in submerged culture.
Auricularia auricula-judae
Key points
- Exo-polymer (EP) was produced at 1.2 g l(-1) in submerged culture of Auricular auricula-judae
- Crude EP (AJ-0) has 70% anti-complementary activity (inhibition of total complementary hemolysis 50%; ITCH50)
- The activating pathway of the complement system occurred through both the classical and alternative pathways, though the major pathway was the classical one
- Fractionation of AJ-0 using Sepharose CL-6B gel chromatography gave three major fractions (AJ-Fr-I, II and III) of which the first was the most active
- The mycelial growth and EP production of A. auricula-judae were optimal at pH 6, 25 degrees C and pH 5, 25 degrees C, respectively
Metadata-grounded summary
Citation abstract
Exo-polymer (EP) was produced at 1.2 g l(-1) in submerged culture of Auricular auricula-judae. Crude EP (AJ-0) has 70% anti-complementary activity (inhibition of total complementary hemolysis 50%; ITCH50). The activating pathway of the complement system occurred through both the classical and alternative pathways, though the major pathway was the classical one. Fractionation of AJ-0 using Sepharose CL-6B gel chromatography gave three major fractions (AJ-Fr-I, II and III) of which the first was the most active. The mycelial growth and EP production of A. auricula-judae were optimal at pH 6, 25 degrees C and pH 5, 25 degrees C, respectively.
Citation
Jeong SC, Cho SP, Yang BK, Gu YA, Jang JH, Huh TL, et al. (2004). Production of an anti-complement exo-polymer produced by Auricularia auricula-judae in submerged culture. Biotechnology letters https://doi.org/10.1023/b:bile.0000025904.21519.3a PMID: 15269542
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