Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes.

Lentinula edodes

Immune supportGut & microbiome

SpeciesShiitake, Hua Gu

JournalBioscience, biotechnology, and biochemistry

Year2017

Key points

Lentinula edodes (shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia
In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene from Pleurotus ostreatus driven by L. edodes laccase 1 gene promoter into L. edodes for expression
The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain

Metadata-grounded summary

Citation abstract

Lentinula edodes (shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia. In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene from Pleurotus ostreatus driven by L. edodes laccase 1 gene promoter into L. edodes for expression. The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain.

Citation

Sato T, Irie T, Yoshino F (2017). Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes. Bioscience, biotechnology, and biochemistry https://doi.org/10.1080/09168451.2017.1332977 PMID: 28585469

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