Maitake, Hen of the Woods · 2017 · Journal Article
High relevanceHeterologous expression of two minor laccase isozyme cDNAs from the edible mushroom Grifola frondosa.
Grifola frondosa
Key points
- Two cDNAs encoding the minor laccase isozymes (Lac2 and Lac3) of Grifola frondosa were cloned, characterized, and expressed in Pichia pastoris
- The recombinant Lac2 (rLac2) was stable at pH 6.0, whereas the recombinant Lac3 (rLac3) was stable in a broad pH range (pH 4.0-8.0)
- In addition, rLac2 and rLac3 showed the highest catalytic efficiency (k cat /K m ) for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Metadata-grounded summary
Citation abstract
Two cDNAs encoding the minor laccase isozymes (Lac2 and Lac3) of Grifola frondosa were cloned, characterized, and expressed in Pichia pastoris. The recombinant Lac2 (rLac2) was stable at pH 6.0, whereas the recombinant Lac3 (rLac3) was stable in a broad pH range (pH 4.0-8.0). In addition, rLac2 and rLac3 showed the highest catalytic efficiency (k cat /K m ) for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid).
Citation
Nitheranont T, Watanabe A, Asada Y (2017). Heterologous expression of two minor laccase isozyme cDNAs from the edible mushroom Grifola frondosa. Bioscience, biotechnology, and biochemistry https://doi.org/10.1080/09168451.2017.1394814 PMID: 29090627
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