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Turkey Tail, Yun Zhi · 2009 · Research Support, Non U.S. Gov'T

Medium relevance

Pyranose 2-oxidase (P2O): production from trametes versicolor in stirred tank reactor and its partial characterization.

Trametes versicolor

OncologyImmune support
SpeciesTurkey Tail, Yun Zhi
JournalPreparative biochemistry & biotechnology
Year2009

Key points

  • Optimization of pyranose-2-oxidase (P2O) production conditions from Trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, pH 5.0, temperature, 26 degrees C, and agitation rate 150 rpm
  • For the first time, P2O production was also carried out in a stirred tank reactor (STR) with 2.2 L working volume in the optimized medium composition, and biomass, P2O activity, protein, nitrogen and glucose concentrations were also monitored besides pH and dissolved oxygen (DO)
  • In the STR, P2O activity peaked on day 9
  • Partial enzyme characterization occurred and optimum pH and temperature were detected as 7.0 and 37 degrees C, respectively
  • K(m) value was found to be 1.009 mM

Metadata-grounded summary

Citation abstract

Optimization of pyranose-2-oxidase (P2O) production conditions from Trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, pH 5.0, temperature, 26 degrees C, and agitation rate 150 rpm. For the first time, P2O production was also carried out in a stirred tank reactor (STR) with 2.2 L working volume in the optimized medium composition, and biomass, P2O activity, protein, nitrogen and glucose concentrations were also monitored besides pH and dissolved oxygen (DO). In the STR, P2O activity peaked on day 9. Partial enzyme characterization occurred and optimum pH and temperature were detected as 7.0 and 37 degrees C, respectively. K(m) value was found to be 1.009 mM.

Citation

Pazarlioglu NK, Akkaya A, Tahsinsoy D (2009). Pyranose 2-oxidase (P2O): production from trametes versicolor in stirred tank reactor and its partial characterization. Preparative biochemistry & biotechnology https://doi.org/10.1080/10826060802589569 PMID: 19090419

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