Optimization of Ganoderma lingzhi triterpene extraction method and its hypoglycemic activity.

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To optimize the continuous reflux extraction process of triterpenes from Ganoderma lingzhi using an orthogonal test and to lay a foundation for its industrial application, an orthogonal experiment was carried out for optimization. The optimal conditions were obtained and verified. The continuous reflux extraction technology for triterpenes from G. lingzhi was optimized. The vanillin-glacial acetic acid method was used to determine the content of triterpenes in G. lingzhi. The optimal process parameters were as follows: an ethanol concentration of 80%, an extraction time of 1.5 hr, and a solid-liquid ratio of 1:26. The average extraction yield was 2.412%, with a relative standard deviation of 1.079%. Taking the inhibition rate of α-glucosidase activity as the index of evaluating hypoglycemic activity, G. lingzhi triterpene has a significant effect on hypoglycemic activity. The optimized triterpene reflux extraction process for G. lingzhi is feasible and stable, providing a scientific reference for the extraction of effective components from G. lingzhi in subsequent stages. The red text indicates deleted content, and the yellow text indicates added content. In order to optimize the continuous reflux extraction process of triterpenes from G. lingzhi through orthogonal testing and to establish a foundation for its industrial application, an orthogonal experiment was conducted for optimization purposes. The optimal conditions were determined and validated. Subsequently, the continuous reflux extraction technique for triterpenes from G. lingzhi was optimized. The vanillin-glacial acetic acid method determined triterpene content. Optimal parameters were: ethanol concentration of 80%, extraction time of 1.5 h, and solid-liquid ratio of 1:26, with an average extraction yield of 2.412% (RSD = 1.079%). Using α-glucosidase activity inhibition rate as an index, G. lingzhi triterpenes showed significant hypoglycemic activity. The optimized extraction process is feasible and stable, offering a scientific reference for subsequent extraction of G. lingzhi 's effective components.