Comparison of chemical profiles, antioxidation, inhibition of skin extracellular matrix degradation, and anti-tyrosinase activity between mycelium and fruiting body of Cordyceps militaris and Isaria tenuipes.

Ophiocordyceps sinensis

Energy & fatigueSkin & hydrationRespiratory

SpeciesCordyceps, Caterpillar Fungus

JournalPharmaceutical biology

Year2022

Key points

Since laboratory cultivation has begun for these fungi, increased output has been achieved
OBJECTIVE: This study compared the chemical profiles, antioxidant, anti-tyrosinase, and skin extracellular matrix degradation inhibition between mycelium and fruiting body of C. militaris and I. tenuipes
MATERIALS AND METHODS: The antioxidative potential of 10% v/v aqueous infused extract from each fungus was separately investigated using 2,2-azinobis(3-ethylbenzo-thiazoline-6-sulphonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant ability, and ferric thiocyanate methods
The inhibition against MMP-1, elastase, and hyaluronidase were determined to reveal their anti-wrinkle potential
The extract from I. tenuipes fruiting body (IF) was highlighted as an extraordinary elastase inhibitor (IC 50 = 0.006 ± 0.004 mg/mL), hyaluronidase inhibitor (IC 50: 30.3 ± 3.2 mg/mL), and antioxidant via radical scavenging (ABTS IC 50: 0.22 ± 0.02 mg/mL; DPPH IC 50: 0.05 ± 0.02 mg/mL), thereby reducing ability (EC 1: 95.3 ± 4.8 mM FeSO 4 /g extract) and lipid peroxidation prevention (IC 50: 0.40 ± 0.11 mg/mL)
IF had a three-times higher EC 1 value than ascorbic acid and significantly higher elastase inhibition than epigallocatechin gallate

Metadata-grounded summary

Citation abstract

CONTEXT: Cordyceps militaris and Isaria tenuipes (Cordycipitaceae) are high-value fungi that are used for health-promoting food supplements. Since laboratory cultivation has begun for these fungi, increased output has been achieved.

OBJECTIVE: This study compared the chemical profiles, antioxidant, anti-tyrosinase, and skin extracellular matrix degradation inhibition between mycelium and fruiting body of C. militaris and I. tenuipes.

MATERIALS AND METHODS: The antioxidative potential of 10% v/v aqueous infused extract from each fungus was separately investigated using 2,2-azinobis(3-ethylbenzo-thiazoline-6-sulphonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant ability, and ferric thiocyanate methods. The inhibition against MMP-1, elastase, and hyaluronidase were determined to reveal their anti-wrinkle potential. Anti-tyrosinase activities were determined.

RESULTS: C. militaris and I. tenuipes extracts were found to contain a wide range of bioactive compounds, including phenolics, flavonoids, and adenosine. A correlation was discovered between the chemical compositions and their biological activities. The extract from I. tenuipes fruiting body (IF) was highlighted as an extraordinary elastase inhibitor (IC 50 = 0.006 ± 0.004 mg/mL), hyaluronidase inhibitor (IC 50: 30.3 ± 3.2 mg/mL), and antioxidant via radical scavenging (ABTS IC 50: 0.22 ± 0.02 mg/mL; DPPH IC 50: 0.05 ± 0.02 mg/mL), thereby reducing ability (EC 1: 95.3 ± 4.8 mM FeSO 4 /g extract) and lipid peroxidation prevention (IC 50: 0.40 ± 0.11 mg/mL). IF had a three-times higher EC 1 value than ascorbic acid and significantly higher elastase inhibition than epigallocatechin gallate.

DISCUSSION AND CONCLUSIONS: IF is proposed as a powerful natural extract with antioxidant and anti-wrinkle properties; therefore, it is suggested for further use in pharmaceutical, cosmeceutical, and nutraceutical industries.

Citation

Prommaban A, Sriyab S, Marsup P, Neimkhum W, Sirithunyalug J, Anuchapreeda S, et al. (2022). Comparison of chemical profiles, antioxidation, inhibition of skin extracellular matrix degradation, and anti-tyrosinase activity between mycelium and fruiting body of Cordyceps militaris and Isaria tenuipes. Pharmaceutical biology https://doi.org/10.1080/13880209.2021.2025255 PMID: 35068295

Open citation