A novel polysaccharide from Pholiota nameko (T. Itô) S. Ito & S. Imai residue: chemical structure and hypoglycaemic activity in vitro.

Pholiota microspora

Immune support

SpeciesNameko, Nametake

JournalNatural product research

Year2025

Key points

A novel polysaccharide (APNP) with a molecular weight of 3.56 × 10 3 KDa and a total sugar content of 92.35 ± 0.07% was isolated from Pholiota nameko (T. Itô) S. Ito & S. Imai residue using alkaline extraction
Monosaccharide composition analysis revealed that APNP consisted of galactose, glucose, and mannose
FT-IR and NMR analysis results indicate that APNP form linkages between (1→3), (1→6), and (1→3,6)-β-D-glucose, (1→)-α-D-glucose, (1→)-α-D-galactose, and (1→3,6)-β-D-mannose
Thermogravimetric analysis revealed three distinct pyrolysis phases in APNP. Congo Red assay results indicated the absence of triple-helical conformation in APNP under weakly alkaline conditions
In vitro assays demonstrated α-glucosidase inhibitory activity of APNP, with an IC 50 value of 0.165 mg/mL. HepG2-IR insulin resistance model and Western blot analyses demonstrated APNP's ability to ameliorate insulin resistance and enhance glucose metabolism

Metadata-grounded summary

Citation abstract

A novel polysaccharide (APNP) with a molecular weight of 3.56 × 10 3 KDa and a total sugar content of 92.35 ± 0.07% was isolated from Pholiota nameko (T. Itô) S. Ito & S. Imai residue using alkaline extraction. Monosaccharide composition analysis revealed that APNP consisted of galactose, glucose, and mannose. FT-IR and NMR analysis results indicate that APNP form linkages between (1→3), (1→6), and (1→3,6)-β-D-glucose, (1→)-α-D-glucose, (1→)-α-D-galactose, and (1→3,6)-β-D-mannose. Thermogravimetric analysis revealed three distinct pyrolysis phases in APNP. Congo Red assay results indicated the absence of triple-helical conformation in APNP under weakly alkaline conditions. In vitro assays demonstrated α-glucosidase inhibitory activity of APNP, with an IC 50 value of 0.165 mg/mL. HepG2-IR insulin resistance model and Western blot analyses demonstrated APNP's ability to ameliorate insulin resistance and enhance glucose metabolism.

Citation

Zhang X, Geng X, Wang M, Li T, Wang L, Cui C, et al. (2025). A novel polysaccharide from Pholiota nameko (T. Itô) S. Ito & S. Imai residue: chemical structure and hypoglycaemic activity in vitro. Natural product research https://doi.org/10.1080/14786419.2025.2598059 PMID: 41363254

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