Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli.

Cordyceps militaris

Energy & fatigue

SpeciesCordyceps, Scarlet Club

JournalBioscience, biotechnology, and biochemistry

Year2023

Key points

Ascomycete lectins may play an important role in their life cycle
In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search
Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin

Metadata-grounded summary

Citation abstract

Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin.

Citation

Ono A, Zhang J, Tanaka Y, Sato C, Yoda A, Ogata M, et al. (2023). Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli. Bioscience, biotechnology, and biochemistry https://doi.org/10.1093/bbb/zbad045 PMID: 37073122

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