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Shiitake, Hua Gu · 2026 · Research Support, Non U.S. Gov'T

High relevance

Identification of regulatory candidate genes for Lentinula edodes pileus development based on transcriptome analysis.

Lentinula edodes

Immune supportGut & microbiome
SpeciesShiitake, Hua Gu
JournalG3 (Bethesda, Md.)
Year2026

Key points

  • To elucidate the molecular mechanisms governing pileus development, we conducted comparative transcriptome analysis via RNA sequencing across 3 distinct developmental stages: Early button stage, Young fruiting body stage, and mature fruiting body stage
  • Gene expression profiling revealed a substantial number of differentially expressed genes (DEGs) between stages, with 283 conserved DEGs spanning the entire developmental continuum
  • Systematic mining of these conserved DEGs identified 3 candidate regulatory genes encoding: Alpha-amylase, Heat shock protein 70 (HSP70), Phosphatidylserine decarboxylase
  • Quantitative PCR validation confirmed the accuracy of both RNA-Seq data and DEG identification
  • Enzymatic activity assays demonstrated significant stage-specific variations in: Antioxidant enzyme activities, Membrane lipid peroxidation levels
  • This study provides valuable insights into the molecular framework underlying pileus morphogenesis in Lentinula edodes

Metadata-grounded summary

Citation abstract

The pileus serves as a primary determinant of market grade and commercial value in Lentinula edodes. To elucidate the molecular mechanisms governing pileus development, we conducted comparative transcriptome analysis via RNA sequencing across 3 distinct developmental stages: Early button stage, Young fruiting body stage, and mature fruiting body stage. Gene expression profiling revealed a substantial number of differentially expressed genes (DEGs) between stages, with 283 conserved DEGs spanning the entire developmental continuum. Systematic mining of these conserved DEGs identified 3 candidate regulatory genes encoding: Alpha-amylase, Heat shock protein 70 (HSP70), Phosphatidylserine decarboxylase. Quantitative PCR validation confirmed the accuracy of both RNA-Seq data and DEG identification. Enzymatic activity assays demonstrated significant stage-specific variations in: Antioxidant enzyme activities, Membrane lipid peroxidation levels. This study provides valuable insights into the molecular framework underlying pileus morphogenesis in Lentinula edodes.

Citation

Zhao X, Wu C, You H, Xu Y, Zha X, Song L, et al. (2026). Identification of regulatory candidate genes for Lentinula edodes pileus development based on transcriptome analysis. G3 (Bethesda, Md.) https://doi.org/10.1093/g3journal/jkaf316 PMID: 41482742

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